Justice 4 ALL Madeleine McCann Family
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JOHN ROBERT LOWE BSc CBiol MlBiol RFP

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JOHN ROBERT LOWE BSc CBiol MlBiol RFP

Post by christabel on Mon Jan 18, 2010 5:20 am

This is the ONE
Manual Pages 300-325
PDF ref: 02-volumeII-apensoI-pages18-43
============================================

Witness deposition
(Criminal Procedure Rules, r27.1 (1);
Criminal Justice Act 1967, s.9; Magistrates' Courts Act 1980, s.5B)


Deposition of: JOHN ROBERT LOWE BSc CBiol MlBiol RFP Age: Older than 18

Profession of Witness: Forensic Scientist

Address of Witness:
Forensic Science Service Ltd.,
Birmingham Laboratory, Priory House, Gooch Street North,
Birmingham, B5 6QQ

With respect to:
References FSS: 300 655 190 / 400 947 125
References Client: 07/06085, 201/07.0GALGS
CJS URN:

I declare that:
This deposition (consisting of twenty two pages signed by me) corresponds to the truth, to the best of my knowledge, and I give it knowing that, if it is presented as evidence, I could be subject to [lit: the target of] penal action in the case I had deliberately declared something that I knew to be false or that I thought was not the truth;
I declare also that,
I am expert in the area of forensic sciences and that I was asked to give a deposition. I confirm that I have read the directions [guidance] in the pamphlet Disclosure: Expert's evidence and unused material [Divulgacao: provas periciais e material nao utilizado] that specifies my functions and documents my responsibilities, with respect to the quality of the disclosure of an expert witness. I followed that guidance and understand the ongoing nature of my responsibilities in terms of disclosure. In accordance with my duties of disclosure, as documented in the guidance pamphlet, I:

a. confirm that I complied with my duty to record, to conserve and to divulge material, in accordance with the Law relating to Investigations and the Penal Process, of 1996, as amended;

b. compiled an index of all the material. I assure that such index will be updated in the case additional material is delivered to me or comes forth [emerges];

c. am advised that in the case my opinion changes relative to any determining [decisive/conclusive] question, I am obligated [obliged] to inform the person responsible for the investigation, as soon as possible, presenting him with my reasons.

Signed: [Signature appended]
Data: 18 Junho 2008

FSS-GF-679 Emissao 2, Pagina 1

Qualifications and Experience

I have a degree in Sciences in the areas of Microbiology and Biochemistry. I am a Chartered Biologist [Note: the highest status achievable by a professional biologist], a member of the Institute of Biology, a forensic doctor enrolled in the Order and a forensic scientist in the Birmingham Laboratory of the Forensic Science Service. My area of specialty is the examination of biological evidence including the analysis of spots of body fluid and the interpretation of DNA profile results.

Information

Based on the information furnished by the Leicestershire Constabulary and the Policia Judiciaria, I was informed that, allegedly, on 3 May 2007 Madeleine McCann disappeared from apartment 5A, Ocean Club, na Praia da Luz, Lagos, Portugal.

My examinations, interpretations and conclusions are in line with the statements of information available during the examination. In the case there are alterations relating to that information, I shall have to reconsider the conclusions that I drew in the light of the new circumstances. Additional information will have to be furnished prior to any trial.

Receipt of the objects

Some of the objects that were collected from apartment 5A and from a vehicle, Renault 'Scenic' (matricula 59-DA-27), hired by Gerry e Kate McCann when they were in Portugal. Those objects were delivered to the Forensic Science Service(R) by the Police Science Laboratory.

FSS-GF-679 Emissao 2, Pagina 2

At the same time, reference samples from those Portuguese police officers who had been active in the crime scene were delivered.

The Forensic Science Service(R) received the [above] objects on 7 August 2007, in sealed, secure packages.

On 8 August 2007, the Forensic Science Service(R) received a piece of cloth/cotton wool (object MJN994) from Leicestershire Constabulary. That object was inside a sealed package.

On 12 October 2007, the Forensic Science Service(R) received a blood spot in a cardboard frame (object JRB/1) from Leicestershire Constabulary. That object was inside a sealed package.

Objective

Th objective of the laboratory examination was to examine the presented objects with respect to the presence of blood, cellular material and hair that may prove to be [have been] from Madeleine McCann or one or other member of her family, or from any of the Portuguese police who had been active [working/present] at the crime scene.

Technical questions

Establishment of the DNA profile

In this case two types of DNA analysis were used: the standard technique called SGM+ and a more precise one called Low Copy Number (LCN).

FSS-GF-679 Emissao 2, Pagina 3

Both techniques look at the same regions of DNA, thereby allowing a comparison to be made between DNA profiles obtained through SGM+ with those obtained through LCN.

Establishment of the DNA profile through SGM Plus

DNA is a complex chemical found in almost every cell of the human body. It carries genetic information that determines the physical and chemical characteristics of a person.

DNA analysis uses a technique in which specific regions [areas] are seen and copied (or amplified) many times. A DNA profile obtained from biological material, such as blood, semen, saliva or hair may be compared with a DNA profile obtained from a reference sample of any person. In the case that the DNA profile of the particular person is different from the DNA profile of the biological material, then that person is not the source of that material. If the profiles are equal [match], then that person, together with other persons having the same DNA profile, may be considered as a potential source of the material.

The significance [import] of a match may then be analysed in relation to the probability of obtaining that match by chance.

Establishment of the DNA profile through LCN

i) in this case, the protocols used on samples collected from a crime scene serve, essentially, to increase the precision of the [chemical] reactions in the establishment of the DNA profile and, consequently, to increase the possibility to detect extremely small quantities of DNA in a sample. This is achieved through increasing the number of amplification cycles.

FSS-GF-679 Emissao 2, Pagina 4

ii) These procedures to establish a DNA profile are applied in the laboratory when routine tests that require a minimum quantity of DNA to be extracted have proven to be, or are considered, inappropriate for successful analyses of a specific sample. This may have to [be done with] a small quantity of cellular material available for tests and/or [due to] the condition of the spot [speck], that can affect the quality of DNA and its effective [efficient] amplification.

iii) Increasing the number of amplification cycles has the effect of significantly increasing the sensitivity of the technique, such that, theoretically, only some [a few] cells are necessary for a successful analysis. The profiles generated in this way should [must] be interpreted in relation to the circumstances of the case and, in particular, to the possibility of detection of quantities of vestigial DNA originating from unknown sources.

iv) A observacao de perfis STR mistos (isto e, de mais de um individuo) pode ser prevenida quando estas condicoes se verificam.
TRANSLATOR'S NOTE: This sentence can have several meanings. The literal translation is:
"The observation of mixed STR profiles (that is, from more than one person) may be prevented when those conditions occur."
If, however, the writer is intending to convey a linkage with his previous paragraph, it can be translated as:
"Mixed DNA profiles may be predicted [alternative meaning of 'prevenir': to warn; to forewarn] when those conditions [from the previous paragraph, i.e. the possibility of unknown sources] occur.
We would need to see the actual English report written by Lowe to understand his intended meaning.

[/i]Experimental data is available that permits a scientist to assess some questions relating to the transference and persistance of low-level DNA in objects and if they can be put together in [whether they - the questions/assessments - are relevant to] this case. Hence, consideration must be given to the manner in which the detected DNA was transferred to the object and, consequently, the relevance of the discovery of the corresponding DNA profiles to those individuals concerned.[/i]

TRANSLATOR'S NOTE: The entire paragraph above was fraught with difficulty, mainly because of its relevance and importance when we read several of Lowe's analyses later on in the report. The encountered difficulty raised the question in my mind as to whether the English-to-Portuguese translator, Lieve van Loock, simply converted written words - as I am attempting to do in reverse - or if (s)he actually had access to Lowe in order to question his intended meaning. If the former then (s)he, like me, was faced with the need to decide on an intended meaning before selecting the words to be written in the translation. In other words, I could be attempting to translate a mis-translation of the original English.
Having said that, however, the above difficulty does not detract from the interpretation of what Lowe said in his individual analyses, as we shall see later.

FSS-GF-679 Emissao 2, Pagina 5

v) All the preparations of DNA were done in duplicate, conforming to procedure when protocols pertaining to increased sensitivity are applied. It [this] is required [in order] to attain reproducible results before the strips of DNA observed in the profiles will be considered as a true representation of the DNA present in this [the] sample.

My interpretation(s), conclusions and methods of work took into account all of the above aspects.
[Alternatively: I was mindful of all the above aspects when performing my work, when making my interpretations, and drawing my conclusions.]

In the appendix at the end of this statement additional details are furnished about the procedures used in obtaining the DNA profiles.

Analysis and Results

I performed analyses in this case with the help of scientific support personnel. A list of those people was made in the Record [register] of Forensic Examination, which I present as object [document] JRL/FER/1a. A complete record of work performed, with notes made at the time of the work, exists under the reference 300 655 190. Those notes are available for verification [examination] in the laboratory, if required.

Oral swabs of reference material

CB/1 Gerald McCann
CB/2 Kate McCann (nee Healy)
SBM/2 Amelie McCann
SBM/3 Sean McCann

My colleague, Sarah Vraitch, furnished me with copies of reference DNA profiles of the above individuals. Each of the profiles was different from the others.

FSS-GF-679 Emissao 2, Pagina 6

14 Pedro Vilhena
286/2007CRL22 Fernando Viegas
286/2007CRL23 Lino Henriques
286/2007CRL24 Bruno Antunes

The DNA profiles of these persons were obtained from their respective samples. The profiles were not only different from each other, they were different from those of the McCann family.

Reference sample of blood

JRB/1 Madeleine McCann
From this sample was obtained a DNA reference sample that was different from those of her immediate family, described above. This DNA profile was the same as that obtained from possible spots of saliva existing on the pillowcase (SJM/1).

Objects attributed to the motor vehicle Renault 'Scenic' (matricula 59-DA-27)

286C/2007-CRL1 D Nail ([human] hand)
From this fragment of a nail from the finger of a [human] hand, a DNA result was obtained through the LCN technique which corresponded to Gerald McCann. In the same result an additional DNA component, unique and unconfirmed, was found that left no room for any other interpretation.

FSS-GF-679 Emissao 2, Pagina 7

286C/2007-CRL2E Nail (hand)
From this fragment ... a DNA result was obtained through the LCN technique which corresponded to Kate Healy. In the same DNA result were found two more DNA components, one of which was not confirmed; these DNA components left no room for any other interpretation.

286C/2007-CRL10 Baggage compartment
This object comprised two sections of the baggage compartment of the Renault Scenic, the first being a baggage compartment lined with fabric with ventilation holes (designated in the UK laboratory as CRL/10(1)) and, the second a moulded plastic extension (designated in the laboratory as CRL/10(2)).

A mixed, low-level DNA result, appearing to be from at least three people, was obtained from the cellular material collected (harvested) from the baggage compartment lined with fabric (286C/2007-CRL/10(1)) of the motor vehicle. That sample was submitted to tests to obtain DNA profiles through the LCN technique.

A DNA result through the LCN technique, which appeared to be from at least three persons, was obtained from the cellular material collected (harvested) from the baggage compartment lined with fabric (286C/2007-CRL/10(1)). In my opinion, this result is too complex to make a meaningful interpretation.

FSS-GF-679 Emissao 2, Pagina 8

The attempt to obtain a DNA profile from any cellular material collected from the plastic area on the baggage compartment (286C/2007-CRL /10(2)) was unfruitful [fruitless, in vain, useless, unproductive, unsuccessful], because no DNA profile was obtained.

A mixed, low-level DNA result, that appeared to be from at least two persons, was obtained from a second area of the baggage compartment plastic (286C/2007-CRL /10(2)). This sample was submitted for tests to obtain DNA profiles through LCN.

A DNA result by complex LCN that appeared to be from at least three persons, was obtained from cellular material collected on the section of the baggage compartment 286C 2007 CRL10 (2) area 2. In my opinion, that result is too complex for a meaningful interpretation.

An incomplete, low-level DNA profile that matched corresponding components in the profile of Gerald McCann was obtained from cellular material present on the card key? (286C/2007-CRL (12)). I guess this is the fob of the car-keys

My colleague, Andrew Palmer, submitted various hair collected from the Renault Scenic for tests, using ... LCN.
Those hairs were designated as 7B hair 1 and 7C hairs 7, 13 e 15. Attempts to obtain a DNA profile of each hair by LCN was unfruitful, because no DNA profile was obtained by LCN, possibly due to there being an insufficient quantity of good quality DNA.

FSS-GF-679 Emissao 2, Pagina 9

Objects attributed to the Apartment 5A of the Ocean Club

286/2007-CRL (1) Pieces of floor-tile identified as number 1
A DNA result by LCN, that appeared to be from at least three persons and that was too complex for a meaningful interpretation, was obtained from the cellular material collected from these floor tiles.

286/2007-CRL (2) Pieces of floor-tile identified as number 2
An inconclusive, incomplete DNA result, consisting of only some unconfirmed DNA components, was obtained from the cellular material recovered from the edges of floor-tile 2 from the apartment floor. The attempt to obtain a DNA profile from any cellular material recovered from the larger area of floor-tile 2 was unfruitful, given that it was not possible to obtain any DNA profile. These samples were then subjected to LCN analysis.

An incomplete, low-level DNA result was obtained through LCN from cellular material in an area of floor-tile 2. In my opinion, there is not any positive proof that supports the theory of any of the members of the McCann family to have contributed DNA to this result. A DNA result was obtained through LCN consistent with only one DNA component from a second area of floor-tile 2. In my opinion, this component leaves no room for any other interpretation.

286/2007-CRL (3) Pieces of floor-tile identified as number 3
The attempts to a DNA profile from any cellular material in two areas of this floor-tile were unfruitful, given that no DNA profile was obtained. These samples were submitted for LCN analysis.

FSS-GF-679 Emissao 2, Pagina 10

A DNA result that consisted of only some DNA components was obtained through LCN analysis of cellular material recovered from one area of floor-tile 3. In my opinion, this result left no room for any other interpretation. The attempt made the obtain a DNA profile through LCN from a second area of floor-tile 3 was unfruitful, given that no profile was obtained.

286/2007-CRL (4) Pieces of floor-tile identified as number 4
Low-level DNA results were obtained through LCN from cellular material present in swabs collected from these floor-tiles. In my opinion, this resuly contained information too meagre [scanty/poor] to permit a meaningful comparison.

286/2007-CRL (5) Pieces of floor-tile identified close to number 1
The attempt to obtain a DNA result through the LCN technique from some cellular material present in swabs collected from the marks [spots] 1, 2 and 3 were unfruitful, given that no DNA profile was obtained.

286/2007-CRL (6) Pieces of skirting board identified as number 1
A DNA result that appeared to be from at least three persons and that was too complex to permit a meaningful interpretation was obtained through LCN from these objects.

FSS-GF-679 Emissao 2, Pagina 11

286/2007-CRL (7) Pieces of skirting board identified as number 2
A DNA result that appeared to be from at least three persons and that was too complex to permit a meaningful interpretation was obtained through LCN from cellular material recovered from these objects.

286/2007-CRL (8) Pieces of skirting board identified as numbers 2 and 3
A DNA result that appeared to be from at least three persons and that was too complex to permit a meaningful interpretation was obtained through LCN from cellular material recovered from these objects.

286/2007-CRL (9) Pieces of skirting board identified as number 3
A DNA result that contained information too meagre to permit a meaningful interpretation was obtained through LCN from cellular material present in swabs collected from these objects.

286/2007-CRL (10) Pieces of skirting board identified as number 4
A DNA result that appeared to be from at least three persons and that was too complex to permit a meaningful interpretation was obtained through LCN from cellular material recovered from these objects.

FSS-GF-679 Emissao 2, Pagina 12

286/2007-CRL (11) Pieces of skirting board identified close to number 1
A DNA result that appeared to be from at least three persons and that was too complex to permit a meaningful interpretation was obtained through LCN from cellular material recovered from these objects.

286/2007-CRL (12) Pieces of skirting board identified close to number 4
Low-level DNA results were obtained through LCN from cellular material present in swabs collected from these objects. In my opinion, there exists no proof that supports the theory that any member of the McCann family had contributed DNA to these results.

286/2007-CRL (13) Residual/small fragments in the area of the skirting board.
The attempt to obtain a result through LCN from any cellular material that may have been in these fragments was unfruitful, given that no profile was obtained, possibly due to the absence of sufficient good quality DNA.

286/2007-CRL (14) Dust/small fragments in the area of the floor tiles
This object was not adequate to perform DNA profile tests.

FSS-GF-679 Emissao 2, Pagina 13

286/2007-CRL (15) Cement-glue [grouting] between the floor tiles
The attempt to obtain a result through LCN from any cellular material that may have been in these fragments was unfruitful, given that no profile was obtained, possibly due to the absence of sufficient good quality DNA.

286/2007-CRL (16) Cement-glue [grouting] between the floor tiles identified as number 1
A DNA profile that did not match any [of the five members] of the McCann family was obtained through LCN from cellular material recovered in that area.

286/2007-CRL (17) Cement-glue [grouting] between the floor tiles identified as number 2
A DNA profile that appeared to be from at least two sources was obtained through LCN from cellular material recovered in that area. In my opinion, the major part of the profile matched that of Lino Henriques. Breaking [departing] from the principle, for it to have had a DNA contribution from Lino Henriques then the remaining information in the smaller part of the result is too meagre to permit a meaningful interpretation.

286/2007-CRL (18) Cement-glue [grouting] between the floor tiles identified as number 3
The attempt to obtain a result through LCN from any cellular material that may have been in these fragments was unfruitful, given that no profile was obtained, possibly due to the absence of sufficient good quality DNA.

FSS-GF-679 Emissao 2, Pagina 14

286/2007-CRL (19) Cement-glue [grouting] between the floor tiles identified as number 4
The attempt to obtain a result through LCN from any cellular material that may have been in these fragments was unfruitful, given that no profile was obtained, possibly due to the absence of sufficient good quality DNA.

286/2007-CRL (20) Fragments of floor tile and grouting
The attempt to obtain a result through LCN from any cellular material that may have been in these fragments was unfruitful, given that no profile was obtained, possibly due to the absence of sufficient good quality DNA.

286/2007-CRL (21) Fragments of bush/shrubbery
Insofar as it relates to this sample, the examination performed was aimed at the detection of the possible presence of blood, no trace thereof having been found. Particles from the superficial surface layer were recovered and preserved in a way similar to that of hairs and fibres. In my opinion, the capillary roots were not good quality as they were not adequate to perform DNA profile tests.

286/2007-CRL (22, 23 e 24) Oral swabs for elimination
A new deposition to report the examination of these objects will be presented at a future time along with the DNA profiles obtained to date from all the DNA results relating to this case.

[QUESTION for the forum: Has anyone heard of such a new deposition or overall report - in detail or in summary?]

FSS-GF-679 Emissao 2, Pagina 15

286A/2007-CRL 1A & B Swabs collected from the floor of the apartment
An incomplete DNA result, apparently originating from a male individual but not matching any other profile obtained in this case, was obtained through LCN from the cellular material present in the combined swabs.

286A/2007-CRL 2A & B Swabs collected from the floor of the apartment
A mixed DNA result, apparently originating from at least two people, was obtained through LCN from the cellular material present in the combined swabs. In my opinion, there are no indications that justify [confirm/prove] the theory that any member of the McCann family had contributed DNA to this result.

286A/2007-CRL 3A& B Swabs collected from the floor of the apartment
An incomplete and weak DNA result comprising only some unconfirmed DNA components was obtained from the cellular material present in the dry swab (3A). The attempt to obtain a result from any cellular material that may have been in the same area and present in the wet swab (3B) was unfruitful, given that no profile was obtained. These samples were submitted for LCN tests.

An incomplete DNA result was obtained through LCN from cellular material present in the swab (286A/2007 CRL 3A). The low-level DNA result showed very meagre information indicating more than one person. Departing from the principle that all confirmed DNA components within the scope of this result originated from a single source, then these pointed to corresponding components in the profile of Madeleine McCann; however, if the DNA within the scope of this result originated from more than one person then the result could be explained as being DNA originating from [a mixture of DNA from both] Kate Healy and Gerald McCann, for example. DNA profiles established through LCN are extremely sensitive; it is not possible to attribute this DNA profile to a particular body fluid. nor to determine how or when that DNA was transferred to that area.

FSS-GF-679 Emissao 2, Pagina 16

A low-level DNA result was obtained through LCN from the cellular material present in the swab (286A/2007 CRL 3B). In my opinion, there are no indications that justify [confirm/prove] the theory that any member of the McCann family had contributed DNA to this result.

286A/2007-CRL 4A & B Swabs collected from the wall of the apartment
An incomplete DNA result, apparently originating from a female individual, was obtained through LCN from the cellular material present in the combined swabs. In my opinion, there are no indications that justify [confirm/prove] the theory that any member of the McCann family had contributed DNA to this result.

286A/2007-CRL 5A & B Swabs collected from the wall of the apartment
A mixed DNA result, apparently originating from at least two persons, was obtained through LCN from the cellular material present in the combined swabs. In my opinion, there are no indications that justify [confirm/prove] the theory that any member of the McCann family had contributed DNA to this result. In my opinion, Fernando Viegas could have contributed DNA to this result.

FSS-GF-679 Emissao 2, Pagina 17

286A/2007-CRL 6A & B Swabs collected from the wall of the apartment
The DNA results obtained through LCN from cellular material present in these combined swabs contained information too meagre to permit a meaningful comparison.

286A/2007-CRL 7A & B Swabs collected from the wall of the apartment
A mixed DNA result, apparently originating from at least two persons, was obtained through LCN from the cellular material present in the combined swabs. In my opinion, there are no indications that justify [confirm/prove] the theory that any member of the McCann family had contributed DNA to this result.

286A/2007-CRL 8A & B Swabs collected from the wall of the apartment
The DNA results obtained through LCN from cellular material present in these combined swabs contained information too meagre to permit a meaningful comparison.

286A/2007-CRL 9A & B Swabs collected from the wall of the apartment
An incomplete DNA result, apparently originating from a male individual, was obtained through LCN from the cellular material present in the combined swabs. In my opinion, there are no indications that justify [confirm/prove] the theory that any member of the McCann family had contributed DNA to this result. Also, this result did not match in any way the profile obtained from swabs 286A/2007 CRL 1A & B.

FSS-GF-679 Emissao 2, Pagina 18

286A/2007-CRL 10A & B Swabs collected from the wall of the apartment
A mixed DNA result, apparently originating from at least two persons, was obtained through LCN from the cellular material present in the combined swabs. In my opinion, there are no indications that justify [confirm/prove] the theory that any member of the McCann family had contributed DNA to this result.

286A/2007-CRL 11A & B Swabs collected from the wall of the apartment
The attempt to obtain a DNA result through LCN from all and any cellular material recovered from these combined swabs was unfruitful, given that no profile was obtained, possibly due to the absence of sufficient good quality DNA.

286A/2007-CRL 12A & B Swabs collected from the wall of the apartment
A mixed DNA result, apparently originating from at least two persons, was obtained through LCN from the cellular material present in the combined swabs. In my opinion, there is no evidence that supports the theory that any member of the McCann family had contributed DNA to this result.

FSS-GF-679 Emissao 2, Pagina 19

286A/2007-CRL 13A & B Swabs collected from the wall of the apartment
The attempt to obtain a DNA result through LCN from all and any cellular material recovered from these combined swabs was unfruitful, given that no profile was obtained, possibly due to the absence of sufficient good quality DNA.

286A/2007-CRL 14A & B Swabs collected from the rear of the sofa
Weak and incomplete DNA results consisting only of some unconfirmed DNA components were obtained from the cellular material present in these wet and dry swabs. In my opinion the results are not adequate for comparison purposes. These samples were submitted for LCN analysis.

A mixed, low-level DNA result was obtained through LCN from the cellular material present in each of the swabs. In my opinion, there are no conclusive indications that justify [confirm/prove] the theory that any member of the McCann family had contributed DNA to these results.

FSS-GF-679 Emissao 2, Pagina 20

286A/2007-CRL 15A & B Swabs collected from the rear of the sofa
A weak and incomplete DNA result showing indications as having come from more than one person was obtained from the cellular material present in dry swab (15A) effected on the rear of the sofa. In my opinion the result is not adequate for comparison purposes. These samples were submitted for LCN analysis.

A DNA result, apparently originating from at least three persons of whom at least two were male and contributed the majority of the DNA, was obtained from the cellular material present in the wet swab (15B) effected on the rear of the sofa. In my opinion, there are no conclusive indications that justify the theory that any member of the McCann family had contributed DNA to this result.

An incomplete, low-level DNA result, comprising only some DNA components, was obtained through LCN from the cellular material present in the dry swab (15A). In my opinion, there are no conclusive indications that justify the theory that any member of the McCann family had contributed DNA to this result.

The wet swab (15B) was not submitted for further LCN anaysis.

FSS-GF-679 Emissao 2, Pagina 21

286A/2007-CRL 16 & 16B Two blue curtains and one white curtain
286B/2007-CRL 1 One white section of a curtain

These curtains were analysed for traces of blood, semen and saliva, none of which were detected. The hem of one of the blue curtains (16) was swabbed to collect any cellular material that might exist. An incomplete, inconclusive DNA result consisting only of two unconfirmed DNA components was obtained. In my opinion the result is not adequate for comparison purposes. The sample was submitted for LCN analysis.

An incomplete, low-level DNA result, comprising only some DNA components, was obtained through LCN from the cellular material recovered from the hem of one the curtains. In my opinion, this result contained information too meagre to permit a meaningful interpretation.

Object attributed to Leicestershire Police

HQ MJN/994 Cloth / piece of cotton wool
The object consisted a plastic bag containing a piece of cloth around cotton wool which showed orange and brown spots. No blood was detected.

FSS-GF-679 Emissao 2, Pagina 22

Object sent to Mr & Mrs McCann, Rothley, Leicester
EMJ/53 Envelope

The envelope flap was swabbed to recover any cellular material that might exist. An incomplete, low-level DNA result was obtained through LCN which, in my opinion, was too complex to permit meaningful interpretation.

The attempt to obtain a DNA result through LCN from all and any cellular material that might have existed on the gum of the envelope was unfruitful, given that no DNA profile was obtained.

Conclusion

In my opinion, the laboratory results that were attained did not help to clarify whether or not the DNA results obtained within the scope of this case were from Madeleine McCann.

FSS-GF-679 Emissao 2, Pagina 23
============================
A summary of the results in the report:

Code:
Summary
Total samples/tests in report 64
Sample incomplete 16 times
DNA attempt "unfruitful" 15 times
No support for "theory" 13 times
Sample mixed 10 times
Sample too complex 9 times
Sample low-level 9 times
Sample too meagre 7 times
Named matches 6 times 3xfamily; 2xpolice; 1xMBM
Not tested 3 times WHY not?
Inadequate for testing 2 times
Unmatched profile 1 time Why not followed up?
---------------------------------------
At least 2 people 10 times
At least 3 people 11 times

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Re: JOHN ROBERT LOWE BSc CBiol MlBiol RFP

Post by christabel on Mon Jan 18, 2010 5:23 am

PART 2

TRANSLATION

Report of Investigations regarding a Case Study

This and a report on the results obtained in the framework of the disappearance of
Madeleine McCann, compiled by
JOHN ROBERT LOWE BSc Cbiol MlBiol RFP.

Forensic Science Service Ltd.,
Birmingham Laboratory, Priory House,
Gooch Street North, Birmingham, B5 6QQ

The report consists of two pages and relates to work carried out
scope of the process with the following reference:
References FSS: 300 655 1 90 / 401 020 769
Customer Reference: OP TASK

Date: May 6, 2008

The information contained in this report relates to examinations related to the study
event, held in the framework of the above breakdown. The results
obtained were recorded and maintained in accordance with the guidelines contained in
a booklet called Disclosure: Expert's evidence and unused material. The information
contained in this report is not intended to serve as material evidence. Should it
Submission to the defense of this report or any information
contained therein, means that I may need to provide a statement to
pursuant to Article 9 or 10 of the Criminal Justice Act 1967.

Exm. Detective Superintendent Mr Pryor

This information is intended solely for private purposes and is a
response to the additional request made in the most recent letter rogatory Authorities
Portuguese.

FSS-GF-680 Emissao 2, Page 1

On April 9, 2008, DNA profiles obtained from evidence
286A/2007/CRL1A & B, 4A & B, 9A and 16A & B & B were subjected to an advanced search
the National DNA Database (R).

We obtained several correspondences with the result of the 286A/2007/CRL4A &B;
Most of these were eliminated based on additional information obtained in the framework of the result;
however, keep two (with their bar codes of 90264515 & 90374723).
These profiles were obtained through the old system of SGM, which examines six areas
DNA. For this reason, samples should receive an upgrade to the new system
standard SGMplus. It is probable that these samples are eliminated as possible
taxpayers after the upgrade.

We obtained several correspondences with the result of the 286A/2007/CRL9A &B;
Most of these were eliminated based on additional information obtained in the framework of the result;
however, one keeps (with their bar code 80004801). This is a
correspondence with a sample of a stain recovered in the context of a crime, and not
can now be associated with a particular individual. However, the latter
sample is processed based on the old system of SGM, consider the inconsiderable
significance of the correspondence.

All correspondences obtained for the other samples were eliminated.

If you want more support in this matter, do not hesitate to contact me.

Eminence Dear Sincerely,

[Signature affixed] John Lowe

_________________
Madeleine [Hardcover] Book
Written by Kate (Author)
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christabel
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Re: JOHN ROBERT LOWE BSc CBiol MlBiol RFP

Post by christabel on Mon Jan 18, 2010 5:24 am

TRANSLATION

Report of Examinations Referred to in the Case Study

This is a report about the results obtained within the scope of the disappearance of Madeleine McCann as compiled by
JOHN ROBERT LOWE BSc Cbiol MlBiol RFP.

Forensic Science Service Ltd.,
Birmingham Laboratory, Priory House,
Gooch Street North, Birmingham, B5 6QQ

The report comprises two pages and talks about work performed within the scope of of the case file with the following reference:
References FSS: 300 655 1 90 / 401 020 769
Customer Reference: OP TASK

Date: May 6, 2008

The true information in this report talks about examinations referring to a case study done within the scope of the case file noted above. The respective results obtained were recorded and conserved in conformance with the guidance in the pamphlet called Disclosure: Expert's evidence and unused material. The true information in this report is not intended to serve as probatory material. Should this report or any of the information contained herein be called for presenation against a defense it is understood that it would be necessary to give a deposition covering under terms of sections 9 and 10 of the Criminal Justice Act 1967.

Dear Detective Superintendent Prior,

This information is exclusive, confidential and constitutive supplementary response to the request contained in the recent Letter rogatory from the Portuguese authorities.

FSS-GF-680 Emissao 2, Page 1

On 9 April 2008, the DNA profiles obtained from the probatory components [objects] 286A/2007/CRL1A & B, 4A & B, 9A and 16A & B & B were submitted with a request to the National DNA Database (R).

Various matches were obtained with the results of 286A/2007/CRL4A &B; the majority of them were eliminated based on additional information obtained from the result, however, two remain (namely, bar codes 90264515 & 90374723). Those profiles were obtained using the old system SGM which examined only six areas of DNA. For this reason the samples should be upgraded [re-tested] using the new standard system SGMplus. It is likely that those samples would be eliminated after the upgrade.

Various matches were obtained with the results of 286A/2007/CRL9A &B; the majority of them were eliminated based on additional information obtained from the result, however, one remains (namely, bar code 80004801). It has matched with a sample of a spot recovered from a crime and can not, at this time, be associated with a specific individual. Nevertheless, the sample that was processed with the old system SGM I consider the match to be of negligible significance.

All matches obtained from other samples were eliminated.

If you wish for more support with this material, do not hestitate to contact me.

Yours faithfully
[Signature] John Lowe

_________________
Madeleine [Hardcover] Book
Written by Kate (Author)
Pre Order your copy
http://www.amazon.co.uk/Madeleine-Kate-McCann-Gerry/dp/0593067916/ref=sr_1_3?s=books&ie=UTF8&qid=1289936443&sr=1-3


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